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Benefits: As a UW employee, you will enjoy generous benefits and work/life programs. For a complete description of our benefits for this position, please visit our website.
Located at the University of Washington in Seattle, the Institute for Protein Design (IPD) is a unique interdisciplinary environment where world-class researchers create new biomolecules. Our mission is to create proteins that solve modern challenges in medicine, technology, and sustainability. Joining our team means becoming part of one of the largest and most innovative public universities in the world.
POSITION PURPOSEAs a member of our Core R&D Labs, you will make important research contributions that accelerate progress in protein design. You will initially join the Biologics, Vaccines, Production, and Process Development Research Core.
DUTIES AND RESPONSIBILITIES
80%) Work with other Core R&D Lab members to accomplish research goals. This includes:Produce proteins utilizing microbial systems with standard production techniques.Produce proteins utilizing mammalian cell systems with standard production techniques.Purification of proteins using high-through autosamplers in conjunction with AKTA FLPCs.Characterize proteins using biophysical techniques such as SDS-PAGE, Western Blot, UV-Vis, Dynamic Light Scattering (DLS), High Performance Liquid Chromatography (HPLC), and Electron Microscopy (EM).Review, analyze, and troubleshoot all experimental results.Document protocols run in the wet lab.Follow standard operating procedures and working guidelines.Mentor IPD researchers on Core-specific workflow.Work directly with Principal Investigators, postdoctoral fellows, graduate students, and laboratory staff in assisting with and conducting experiments.10%) Maintain databases and inventories. This includes:Preparation and maintenance of standard laboratory stocks and reagents necessary to research workflow.Maintain inventory for all chemicals, supplies, reagents, and biological samples.Maintain and troubleshoot laboratory equipment.Work with Laboratory Manager to assist with laboratory-wide EH&S compliance.5%) Assist with managing undergraduate research assistants.5%) Present scientific data, attend meetings and team-building events.MINIMUM REQUIREMENTS
- Bachelor's Degree in Biochemistry, Molecular Biology, or any other relevant field and one year experience with protein expression and characterization using biophysical techniques, OR equivalent combination of education/experience.
ADDITIONAL REQUIREMENTS
- Experience with protein expression in microbial systems like E.coli using IPTG and autoinduction.
- Development and optimization of purification methods for gravity and FPLC assisted IMAC, IEX.
- Characterize proteins using biophysical techniques by SDS-PAGE, Western Blot, UV-Vis, DLS, HPLC and EM.
- Strong communication and organization skills and great attention to detail, and ability to thrive in both individual tasks and team environments/assignments.
- A willingness to acquire new skills and learn on the job.
DESIRED QUALIFICATIONS
- The ideal candidate will be someone who has had one (1) or more years of relevant protein biochemistry experience in a laboratory setting in addition to standard chemistry/biochemistry laboratory courses during their undergraduate degree. Hands-on experience with protein purification systems and equipment is highly desirable.
Committed to attracting and retaining a diverse staff, the University of Washington will honor your experiences, perspectives and unique identity. Together, our community strives to create and maintain working and learning environments that are inclusive, equitable and welcoming.
To request disability accommodation in the application process, contact the Disability Services Office at 206-###-#### or ...@uw.edu.
Applicants considered for this position will be required to disclose if they are the subject of any substantiated findings or current investigations related to sexual misconduct at their current employment and past employment. Disclosure is required under Washington state law.
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