Sr. Research Associate - Synthetic Biology
: Job Details :

Summary:

Our client is searching for a Sr. Research Associate for their Synthetic Biology Team.

The synthetic biology team is a R&D task force for biotech and pharmaceutical clients across a wide variety of disease indications and therapeutic modalities in the cell and gene therapy space. The organization uses DNA, RNA, and protein engineering paired with massively parallel screening to create technologies that enhance the performance, precision, and safety of cell, gene, and nucleic acid therapies. The Sr. RA will join the synthetic biology team to contribute to the development of technologies for our external partners as well as the expansion of the organization's SynBioplatform.

The research associate will evaluate the function of genetic elements (such as promoters, UTRs, and synthetic switches), often assisting with novel assay development, brainstorming, and troubleshooting.

Responsibilities & Requirements

• Design, troubleshoot, and optimize new assays and protocols for a variety of cell types to measure expression, function and/or cell phenotypes
• Clone libraries of DNA, RNA, or protein variants
• Prepare samples for NGS
• Screen libraries of variants in primary human cells and immortalized cell lines
• Analyze and record data and provide advice on project future directions
• Present work internally and potentially externally.

Technical Skills & Qualifications

• Mammalian cell culture including experience culturing primary human immune cells and transfecting/transducing multiple types of cells
• GraphPad Prism (fluency)
• Lentiviral production (titering) (transfection), and harvest/purification,
• Expertise cloning libraries and preparing samples for NGS
• Mammalian cell assays including ELISA and multicolor flow cytometry
• Molecular Biology (e.g. PCR, cloning)