Location: Cambridge,MA, USA
MUST HAVES:
Daily Responsibilities/Job Description:
• Solve problems with End-User instrument operations and improve their skills in all aspects of instrument function.
• Provide experiment setup and aid resolve experiment setup, design, and operation issues
• Performs guidance with routine, moderate, and complex analysis for assay specimens in accordance with assay specific gating guides (GG) and standard operating procedures (SOPs)
• Perform routine maintenance on equipment, including QC
• Participate in routine quality investigations, and process effectiveness verifications
• Responds to queries, communications, and workflows with labs to ensure consistency and timely resolution
• Contributes to troubleshooting and process improvements to increase data quality, lower costs, or reduce turnaround times
• Assist with Maintaining laboratory stocks of supplies and reagents
• Become familiar with relevant software used in the facility and assist facility users in learning to use this software
• Demonstrates appropriate use of relevant equipment after training
• Understand common and unique biological applications, cell handling techniques, and reagents used by researchers
• Maintain training and compliance in areas of health and safety, security, environmental and operational aspects of daily activities in the working environment
Qualifications/years of experience:
• BA/BS degree in physical or biological science or a related science and 3 or more years of recent, directly related work experience OR
• Associate's Degree in life science or other relevant discipline and 5 years' Assay Development experience in a laboratory environment OR
• High School Degree/GED with 10 years' Assay Development experience in a laboratory environment.
Must Haves:
• Demonstrated ability to optimize and execute immunoassays, such as MSD/ELISA/Luminex, and multi-color flow cytometry.
• Experience with biospecimen processing, including handling and processing of blood, primary cells, and/or tissues.
• Experience with the development of in vitro cellular assays.
• Understanding of how the flow cytometer and FACS instruments work.
• Willingness to learn from others, taking initiative and being open to iteration.
• Ability to accurately and efficiently execute laboratory protocols and standard operating procedures (SOPs).
• Dedication to diligent record-keeping to ensure accurate documentation of experiments and results.
• Attention to detail and understanding of the basic laboratory environment
• Excellent organizational skills; ability to troubleshoot and solve problems independently
• Ability to follow oral and written directions
Additional Requirements:
• Proficiency in using software tools such as Prism, FlowJo, Microsoft Office, and Electronic Laboratory Notebooks (ELN) for data analysis and documentation.
• Experience or knowledge in immunology research is desirable.
• Support and troubleshoot hardware and software on liquid handlers, bulk dispensers, plate readers and other integrated automated systems
• Experience with Operating and maintaining the lab equipment a plus
• Three or more years of experience with one or more of the following instrumentation systems: Sony SH800 FACS, BD FACSAria / BD Fusion, BD Symphony, Cytek Aurora, ACEA Novocyte
• Experience in working with and/or designing flow cytometry panels (